Composite

Part:BBa_K4156110:Design

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)


pPepT-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 723
    Illegal AgeI site found at 835
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pPepT-mRFP uses the classical red fluorescent protein ( BBa_E1010 ) and the rrnB T1 and T7Te dual terminator ( BBa_B0015 ) to inter-assemble with the pPepT promoter ( BBa_K4156110 ) and introduces the RiboJ sequence as well as RBS B0034 after pPepT to optimize gene expression.


Source

Experimental constructed.

References